Abstract: Eukaryotic genomic DNA and histones together form nucleosomes, but eukaryotic genome DNA can bind a series of enzymes during gene transcription, replication and DNA repair, all can be regulated by ATP-dependent nucleosome remodeling complex． Chromatin remodeling complex INO80 promotes delayed restart of replication forks, and helps transcribed genes to clear RNA polymerase Ⅱ after replication forks collide with the transcript． In addition, INO80 is involved in repairing DNA damage and inhibiting inappropriate transcription at the promoter in the direction opposite to the coding sequence． The basis of these different functions is the core ATPase subunit,which can promote sliding of nucleosomes and exchange of histone variants． We review here the composition of INO80 and its roles in the regulation of gene expression, to gain insights on its biological functions and roles in development, health and disease．