Abstract: White-tailed sea eagle (Haliaeetus albicilla), a large raptor belonging to Accipitridae of Accipitriformes, is a first-class national protected animal in China． This top predator plays a significant role in maintaining ecosystem balance and biodiversity． During the winter, a large wintering population is present in Jingxin Wetland in Hunchun city, Jilin province． To study the population genetics of white-tailed sea eagles, it is necessary to develop microsatellite SSR (simple sequence repeat) and sex identification primers． In this study, microsatellite and sex identification primers were designed based on the whole genome data of white-tailed sea eagle (NCBI), and verified with white-tailed sea eagle fecal samples collected from Jingxin Wetland． A total of 9 pairs of SSR primers and 2 pairs of sex identification primers were confirmed, which will be useful for future studies of the white-tailed sea eagle．