Abstract: The “production-living-ecological” space has occupied a main position for human activities in the terrestrial surface system, and their reasonable planning and use can promote the development of harmony of human-land relations.. Guangdong Province is at the forefront of China’s reform and opening up, but it is also a potential area for spatial conflicts in the “production-living-ecological”. The article first conducted spatial classification and identification of the “production-living-ecological” space in Guangdong Province, studied the evolutionary characteristics from 2000 to 2020, quantified the spatiotemporal evolution of spatial conflicts, and explored the influencing factors at different scales.The results show that 1) among the “production-living-ecological” space in Guangdong Province, the area of ecological space remains the largest, but in the past 20 years, the evolution of living space, production space, and ecological space has become dominant successively; (2) The overall degree of spatial conflict among the “production-living-ecological” space in Guangdong Province is relatively low, with the characteristic of upper spatial clustering; 3) Overall, NDVI, DEM, night light, slope, population, gross domestic product, and temperature have relatively strong explanatory power for the spatial conflicts of “production-living-ecological” space;4) At the local scale, between 4-30km, the effects of NDVI and slope on the spatial heterogeneity of “production-living-ecological” spatial conflicts are significant. As the research scale increases, the effects of population and temperature gradually increase.