AMF对镉胁迫下虎尾草生长、生理特性及镉积累的影响

Effects of AMF on the growth, physiological characteristics and Cd accumulation of Chloris virgata under Cd stress

  • 摘要: 以虎尾草(Chloris virgata Sw.)为宿主植物,根内根孢囊霉和摩西斗管囊霉为菌剂,采用温室盆栽的方式进行镉污染模拟试验,研究丛枝菌根真菌(AMF)对镉胁迫下虎尾草生长、生理特性以及镉积累的影响.主要结果表明:1)未接种AMF时,低浓度镉(5 mg·kg−1)能促进虎尾草的生长,高浓度镉(50 mg·kg−1)则抑制其生长;随着Cd2+浓度增加,虎尾草的抗氧化酶活性(POD)、根系活力呈先上升后下降的趋势,可溶性糖、可溶性蛋白、叶绿素和丙二醛的含量呈上升趋势;2)无镉胁迫时,AMF促进了虎尾草的生长,虎尾草的POD、可溶性糖含量增加;3)所有镉胁迫处理下虎尾草地下部镉浓度大于地上部;接种AMF后,虎尾草地上镉富集系数均增强;4)在一定镉浓度范围内,AMF提高了虎尾草的根系活力、POD、光合色素含量,增强了虎尾草对镉的耐受性.本研究结果可为土壤镉污染修复提供一定的实验依据.

     

    Abstract: Using Chloris virgata. as the host plant and Rhizoctonia solani and Aspergillus niger as mycorrhizal agents, a simulation experiment of cadmium pollution was conducted in greenhouse pots to study the effects of Arbuscular Mycorrhizal Fungi (AMF) on the growth, physiological characteristics, and cadmium accumulation of C. tigris. under cadmium stress. The main results showed that: 1) when not inoculated with AMF, low concentrations of cadmium(5mg/kg) promoted the growth of C. tigris, while high concentrations of cadmium (50 mg/kg)inhibited its growth; with the increase of Cd2+ concentration, the antioxidant enzyme activity (POD) and root vigor of C. tigris showed a tendency to increase and then decrease, and the contents of soluble sugars, soluble proteins, chlorophyll, and malondialdehyde showed a tendency to rise; 2) without cadmium stress, AMF promoted the growth of C. tigris, and the contents of POD and soluble sugar of C. tigris increased; 3) under all cadmium stress treatments, the cadmium concentration of C. tigris was greater than that of the aboveground; after the inoculation of AMF, the aboveground cadmium concentration coefficients of C. tigris were all enhanced; 4) within a certain cadmium concentration range, AMF increased the root vitality of C. tigris, the contents of POD and photosynthetic pigments, and enhanced the tolerance of C. tigris to cadmium. The results of this study can provide a certain experimental basis for the remediation of soil cadmium pollution.

     

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