To investigate the expression and prognostic significance of microtubule-associated serine/threonine kinase 1 (MAST1) in glioma tissues. MAST1 mRNA expression in tumor tissues and prognosis of patients with gliomas were analyzed by the GEPIA 2 database. mRNA seq_325 and mRNA seq_693 transcriptome data and clinical data were obtained from the Chinese Glioma Genome Atlas (CGGA) database, and the differential expression of MAST1 mRNA in glioma tissues with different clinical features was analyzed. The impact of MAST1 expression on the prognosis of patients with glioma was evaluated using Kaplan-Meier survival curves and multivariate Cox regression analysis. The potential signaling pathways involved in MAST1 was

explored by Gene Set Enrichment Analysis (GSEA) software. The expression of MAST1 mRNA was low in glioma tissues. MAST1 mRNA expression was correlated with patient’s age, WHO grade, IDH mutation, and 1p/19q co-deletion status. Low expression of MAST1 was associated with poor prognosis in glioma patients. Further enrichment analysis revealed the involvement of MAST1 in multiple biological processes. It is evident that MAST1 is a potential indicator for prognostic evaluation in glioma patients, and this study provides a theoretical basis for understanding the pathogenesis of glioma.