Abstract: One of the most important ways to improve the quality of undergraduate laboratory teaching and talent cultivation is to timely transform the cutting-edge theories and research techniques of cell biology into experimental teaching. Based on the knowledge of tunneling nanotubes (TNT), this experiment is designed as a comprehensive and exploratory experimental teaching content, including the establishment of serum starvation model and tunneling nanotubes induction, observation of tunneling nanotubes structure by staining of cell microfilaments and nuclei, and detection of cell cycle by flow cytometry. Students firstly establish serum starvation model by mapping the serum concentration for different types of cells, then use cell crawler for cell fluorescence staining, and observe the bridge-like TNT structure under the fluorescence microscope; finally, flow cytometry is used to detect the change of the proportion of G1 cells in the cell cycle in starvation state. Teaching practice shows that the experiment helps to cultivate students' hands-on and scientific research thinking and stimulate students' interest in exploration.