Abstract: The Yangtze River economic belt, as a strategic area with dense population, active economy and sensitive ecology in China, poses a major challenge to the sustainable development of the basin due to land use conflicts. Based on the perspective of landscape pattern and functional coordination, this study constructed a LUC framework for land use conflict identification, analyzed the spatio-temporal differentiation characteristics of land use conflicts in the Yangtze River economic belt from 2005 to 2020, and adopted the spatio-temporal geographic Weighted Regression (GTWR) model to explore its influencing factors. The results show that: 1) From 2005 to 2020, the inter-provincial differences in LUC of the Yangtze River economic belt were significant, presenting a gradient pattern of "high in the east and west, low in the middle, and concentrated along the river". Moreover, the upper reaches were mainly characterized by structural conflicts, while the lower reaches focused on functional conflicts. 2) The LUC of the Yangtze River Economic Belt shows an upward trend, and the center of gravity shifts towards the northeast. The conflicts between the Chengdu-Chongqing urban agglomeration and the Yangtze River Delta region have intensified, but some conflicts in the mountainous areas of southwest China have eased to some extent. 3) The annual average temperature, slope and urbanization rate are the main positive correlation factors, while elevation and sewage treatment rate are the main negative correlation factors. 4) The dominant driving factors of LUC in the Yangtze River economic belt have spatial differences.Based on this, strategies such as regional differentiated regulation and control, dynamic monitoring and inter-provincial coordination are proposed to provide a scientific basis for the rational utilization and sustainable development of land resources in the Yangtze River economic belt.