崔金龙, 童银栋, 赵锋, 麦富源, 李胜楠, 李明月, 王洁, 孙学军, 张强弓. 基于高通量测序技术的西藏冰湖真核浮游植物群落组成特征分析[J]. 北京师范大学学报(自然科学版), 2023, 59(2): 218-229. DOI: 10.12202/j.0476-0301.2022346
引用本文: 崔金龙, 童银栋, 赵锋, 麦富源, 李胜楠, 李明月, 王洁, 孙学军, 张强弓. 基于高通量测序技术的西藏冰湖真核浮游植物群落组成特征分析[J]. 北京师范大学学报(自然科学版), 2023, 59(2): 218-229. DOI: 10.12202/j.0476-0301.2022346
CUI Jinlong, TONG Yindong, ZHAO Feng, MAI Fuyuan, LI Shengnan, LI Mingyue, WANG Jie, SUN Xuejun, ZHANG Qianggong. Eukaryotic phytoplankton community composition in Tibetan glacial lakes analyzed by high throughput sequencing[J]. Journal of Beijing Normal University(Natural Science), 2023, 59(2): 218-229. DOI: 10.12202/j.0476-0301.2022346
Citation: CUI Jinlong, TONG Yindong, ZHAO Feng, MAI Fuyuan, LI Shengnan, LI Mingyue, WANG Jie, SUN Xuejun, ZHANG Qianggong. Eukaryotic phytoplankton community composition in Tibetan glacial lakes analyzed by high throughput sequencing[J]. Journal of Beijing Normal University(Natural Science), 2023, 59(2): 218-229. DOI: 10.12202/j.0476-0301.2022346

基于高通量测序技术的西藏冰湖真核浮游植物群落组成特征分析

Eukaryotic phytoplankton community composition in Tibetan glacial lakes analyzed by high throughput sequencing

  • 摘要: 通过18S rRNA基因V4可变区对西藏廓琼岗日冰川流域3种不同类型冰湖的表层和底层水体进行测序分析,研究冰湖水体真核浮游植物多样性.冰湖中用于注释的序列数碱基占比>97%,表明测得的数据准确可靠.分析结果表明:1)冰湖浮游植物以绿藻门和隐藻门为优势类群,绝对优势种为绿藻门的Chlamydomonadaceae_sp.(0.127)、Chlorophyta(0.112);2)廓琼岗日冰湖水体整体呈碱性,浊度高且范围广,水温偏低,营养盐和叶绿素a水平偏低,湖泊整体处于贫营养状态;3)同一冰湖不同点位和不同类型冰湖的浮游植物优势种和群落具有显著差异,冰川融水补给源和太阳辐射的空间差异可能是导致这种差异的重要因素;4)冰湖浮游植物优势种和群落与青藏高原大型湖泊具有较大差异,较国外高寒小型湖泊具有一定的相似性,表明浮游植物的整体分布及优势种变化在高寒山区小型湖泊水体变化较为一致.研究为调查和认识高海拔冰湖浮游植物群落结构和分布特征提供方法探索和基础信息,为高海拔冰湖水生态环境研究和保护提供依据.

     

    Abstract: Phytoplankton is important for the primary productivity of natural waters.In the context of global climate changes, glacial lakes in high altitude regions are increasing and expanding, community structure of phytoplankton in such glacial lakes undergo rapid changes.High throughput sequencing offers simplicity, rapidity and low error rates, therefore could be used to investigate and analyze distribution and changes of phytoplankton in glacial lakes.The surface and bottom water from three different glacial lakes in the Kuoqionggangri Glacier Basin, Tibet, were sequenced and analyzed in this study.The variable region in 18S rRNA gene V4 was used to study diversity of eukaryotic phytoplankton in glacial lakes.Number of bases used for annotation accounted for > 97%, so measurements were accurate and reliable.Chlorophyta and Cryptophyta were found to be dominant in the glacial Lakes.The absolutely dominant species were found to be Chlamydomonadaceae_sp. (0.127) and Chlorophyta (0.112).The water body in Kuoqionggangri Glacial Lake was alkaline; with high turbidity and wide range, low water temperature, low levels of nutrients and chlorophyll a, the lake is in poor nutrition status.Dominant species and communities of phytoplankton at different sampling points in the same glacial lake, and in different glacial lakes varied markedly, possibly due to differences in glacial meltwater recharge and solar radiation.Dominant species and communities of phytoplankton in glacial lakes were quite different from large lakes on the Tibetan Plateau, but similar to small alpine lakes abroad.Therefore overall distribution and changes in dominant species of phytoplankton were more consistent in small lakes in alpine mountains.This study furthers investigation and understanding of phytoplankton community in high-altitude glacial lakes.

     

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